Composition of Rat Liver Triacylglycerols and Diacylglycerols

Elevation of liver diacylglycerols and molecular species of diacylglycerols in rats fed a lipogenic diet.

The concentration of diacylglycerols in rat liver was measured by a newly developed procedure that entailed a) freeze-clamping of the liver in situ, b) lipid extraction with dimyristoylglycerol as an internal standard, c) thin-layer chromatography, and d) gas-liquid chromatography. Molecular species of diacylglycerol and total diacylglycerols were quantified. The average level of diacylglycerol...

Ribonucleic Acid Composition of Rat Liver Tumor

Studies on normal tissues have suggested that the ribonucleic acid (RNA) of nuclei, mitochon dna, microsomes, and supernatant fluid isolated from tissue homogenate has a characteristic nu cleotide composition (7, 8, 16), while other data did not support such a view (6). Results obtained in this laboratory revealed, in fact, the occurrence of characteristic ribonucleic acids in rat liver frac ti...

The lipid composition of rat liver.

In a previous publication (Getz & Bartley, 1961) the fatty acid compositions of various intracellular compartments of the rat liver were investigated. This work has now been extended to the fatty acid composition ofthe different lipid classes ofthe liver. The data obtained will be used as a basis for comparison of the lipid composition of each of the cell components. This paper reports a compos...

Molecular species of diacylglycerols and phosphoglycerides and the postmortem changes in the molecular species of diacylglycerols in rat brains.

The molecular species of 1,2-diacyl-sn-glycerol (DAG), phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), phosphatidylinositol (PI), phosphatidylinositol 4-phosphate (PIP), and phosphatidylinositol 4,5-bisphosphate (PIP2) from brains of adult rats (weighing 150 g) were determined. The DAG, isolated from brain lipid extracts by TLC, was benzoylated, and the molecul...

Studies on Phospholipids. 9. the Composition of Rat-liver Lecithins.